Gut microbes encourage specialized cells to prune back extra connections in brain circuits that control social behavior, new UO research in zebrafish shows. Pruning is essential for the development of normal social behavior.
The researchers also found that these ‘social’ neurons are similar in zebrafish and mice. That suggests the findings might translate between species—and could possibly point the way to treatments for a range of neurodevelopmental conditions.
“This is a big step forward,” said UO neuroscientist Judith Eisen, who co-led the work with neuroscientist Philip Washbourne. “It also sheds light on things that are going on in larger, furrier animals.”
The team reports their findings in two new papers, published in PLOS Biology and BMC Genomics.
While social behavior is a complex phenomenon involving many parts of the brain, Washbourne’s lab previously identified a set of neurons in the zebrafish brain that are required for one particular kind of social interaction.
Normally, if two zebrafish see each other through a glass partition, they’ll approach each other and swim side by side. But zebrafish without these neurons don’t show interest.
Here, the team found a pathway linking microbes in the gut to these neurons in the brain. In healthy fish, gut microbes spurred cells called microglia to prune back extra links between neurons.
Pruning is a normal part of healthy brain development. Like clutter on a counter, extra neural connections can get in the way of the ones that really matter, resulting in muddled messages.
In zebrafish without those gut microbes, the pruning didn’t happen, and the fish showed social deficits.
“We’ve known for a while that the microbiome influences a lot of things during development,” Washbourne said. “But there hasn’t been a lot of concrete data about how the microbiome is influencing the brain. We’ve done quite a bit to push the boundary there.”
In a second paper, the team identified two defining features of this set of social neurons that may be shared by mice and zebrafish. One is that these cells could be identified by having similar genes turned on—a clue that they might serve similar roles in the brains of both species.
Both gut microbiome disruption and poor neural synapse pruning have been linked to a range of neuropsychiatric conditions like autism spectrum disorder.
“If we can tie these together, it might facilitate better therapeutics for a wide range of disorders,” said Joseph Bruckner, a postdoc in the Eisen and Washbourne labs and the first author on the PLOS Biology paper. His next step is figuring out what molecules are linking the bacteria to the microglia, mapping the pathway between microbes and behavior in even more detail.
Host-associated microbiotas guide the trajectory of developmental programs, and altered microbiota composition is linked to neurodevelopmental conditions such as autism spectrum disorder. Recent work suggests that microbiotas modulate behavioral phenotypes associated with these disorders.
We discovered that the zebrafish microbiota is required for normal social behavior and reveal a molecular pathway linking the microbiota, microglial remodeling of neural circuits, and social behavior in this experimentally tractable model vertebrate.
Examining neuronal correlates of behavior, we found that the microbiota restrains neurite complexity and targeting of forebrain neurons required for normal social behavior and is necessary for localization of forebrain microglia, brain-resident phagocytes that remodel neuronal arbors.
The microbiota also influences microglial molecular functions, including promoting expression of the complement signaling pathway and the synaptic remodeling factor c1q. Several distinct bacterial taxa are individually sufficient for normal microglial and neuronal phenotypes, suggesting that host neuroimmune development is sensitive to a feature common among many bacteria.
Our results demonstrate that the microbiota influences zebrafish social behavior by stimulating microglial remodeling of forebrain circuits during early neurodevelopment and suggest pathways for new interventions in multiple neurodevelopmental disorders.
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